Professor, Mechanobiology Institute, National University of Singapore
+65 6516 8908
Blk S1A, Level 2
Lee Wee Kheng Building
National University of Singapore
14 Science Drive 4
Leader, Mechanotransduction in Tissues
Professor, Department of Biological Sciences, National University of Singapore
Mechanobiology of cells and tissues on viscoelastic surfaces, nanoscale dynamics, biomaging sciences.
The Matsudaira Lab studies the role of mechanics in epithelial tissue migration and remodeling during development and disease. The organization and migration of epithelial tissues are sensitive to the underlying substratum, especially on visco-elastic substrates. This range of stiffness defines the environment of most basement membranes, cell-cell interactions, and early developmental processes. On the one hand, the lab is interested in the mechanical signatures of the development of chirality, convergence, and extension during the early steps in morphogenesis of the Zebrafish embryo. Other studies are focused on the role of substrate viscoelasticity in tissue remodeling during disease and its regulation by cell-matrix and cell-cell signaling pathways. In order to study complex, multi-scale dynamics the lab develops and applies novel imaging methods such as in situ TEM and strain light-sheet microscopy.
Formerly a biology and bioengineering professor at the Massachusetts Institute of Technology (MIT), Paul Matsudaira has studied the cytoskeleton since graduating from college. He was the EM technician of Tom Schroeder where he helped investigate the role of microfilaments in the contractile ring. As a graduate student of Dave Burgess he identified the structure and function of the intestine brush border cytoskeleton. Following postdoctoral research on the assembly of actin bundles at the MPI Biophysical Chemistry with Klaus Weber and the MRC LMB with Alan Weeds, Paul started his academic career at the Whitehead Institute and MIT where his lab studied biophysics of actin and other polymer protein bundles, mechanics of polymers and single cells motility, and developed microanalytical methods. In 2009, he moved to Singapore to establish the Centre for BioImaging Sciences, head the Department of Biological Sciences, and help found the Mechanobiology Institute, National University of Singapore.
PhD in Biological Sciences from Dartmouth College, Hanover, New Hampshire
Selected Publications (5 of 177)
- Zheng J, Han SP, Chiu YJ, Yip AK, Boichat N, Pauli AR, Zhu S, Matsudaira P. 2017 Substrate viscoelasticity induces coalescence of epithelial monolayers via subcellular redistribution of vinculin. Biophys J. 113:1585-98.
- Loh D, Sen S, Bosman M, Tan SF, Zhong J, Nijhuis C, Kral P, Matsudaira P, Mirsaidov U. 2016 Multi-step nucleation of nanocrystals in aqueous solution. Nat. Chem 9:77-82.
- Ai Kia Yip, Keng-Hwee Chiam and Paul Matsudaira. Traction stress analysis and modeling reveal that amoeboid migration in confined spaces is accompanied by expansive forces and requires the structural integrity of the membrane–cortex interactions. Integrative Biology, 2015, DOI: 10.1039/ C4IB00245H. First published online 27 May 2015
- Bhattacharya D, Singh V R, Zhi C, Peter T C, Matsudaira P, Barbastathis G. 2012. Three dimensional HiLo-based structured illumination for a Digital Scanned Laser Sheet Microscopy (DSLM) in thick tissue imaging. Optics Express 20(25):27337-27347.
- Mirsaidov UM, Zheng H, Bhattacharya D, Casana Y, Matsudaira P. 2012. Imaging protein structure in water at 2.7 nm resolution by TEM. Biophys J. 102:L15-7.
- Matsudaira PT, and Verma CS. Editorial. Prog. Biophys. Mol. Biol. 2019;. [PMID: 30951764]
- Wadduwage DN, Singh VR, Choi H, Yaqoob Z, Heemskerk H, Matsudaira P, and So PTC. Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy. Optica 2017; 4(5):546-556. [PMID: 29392168]
- Zheng JY, Han SP, Chiu Y, Yip AK, Boichat N, Zhu SW, Zhong J, and Matsudaira P. Epithelial Monolayers Coalesce on a Viscoelastic Substrate through Redistribution of Vinculin. Biophys. J. 2017;. [PMID: 28844472]
- Zheng JY, Tan HL, Matsudaira PT, and Choo A. Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis. Cell Death Differ. 2017;. [PMID: 28106884]
- . https://www.ncbi.nlm.nih.gov/pubmed/28007915
- Loh ND, Sen S, Bosman M, Tan SF, Zhong J, Nijhuis CA, Král P, Matsudaira P, and Mirsaidov U. Multistep nucleation of nanocrystals in aqueous solution. Nat Chem 2016; 9(1):77-82. [PMID: 27995918]
- Huang X, Huang S, Ong LC, Lim JC, Hurst RJM, Mushunje AT, Matsudaira PT, Han J, and Preiser PR. Differential Spleen Remodeling Associated with Different Levels of Parasite Virulence Controls Disease Outcome in Malaria Parasite Infections. mSphere 2015; 1(1). [PMID: 27303680]
- Shivashankar GV, Sheetz M, and Matsudaira P. Mechanobiology. Integr Biol (Camb) 2015; 7(10):1091-2. [PMID: 26404492]
- Luo Y, Singh VR, Bhattacharya D, Yew EYS, Tsai J, Yu S, Chen H, Wong J, Matsudaira P, So PTC, and Barbastathis G. Talbot holographic illumination nonscanning (THIN) fluorescence microscopy. Laser Photon Rev 2014; 8(5):L71-L75. [PMID: 25678936]
- Mirsaidov U, Mokkapati VRSS, Bhattacharya D, Andersen H, Bosman M, Özyilmaz B, and Matsudaira P. Scrolling graphene into nanofluidic channels. Lab Chip 2013; 13(15):2874-8. [PMID: 23702655]