Pakorn Tony KANCHANAWONG
Associate Professor, Mechanobiology Institute, National University of Singapore
+65 6601 8991 ext 18991
National University of Singapore
5A Engineering Drive 1
Kanchanawong Lab @ NUS
Molecular Mechanics of Mechanotransduction Group
MBInsights Home Science Features News Awards Outreach Events Science Features Molecular controllers of stem cell mechanics How actin regulators interact closely to determine cortex architecture Written [...]
Focal adhesions in embryonic stem cells
Actin remodeling drives bile regurgitation during obstructive cholestasis
Using superresolution imagining to map adherens junction machinery
Reverse engineering molecular machines
Visualizing the building blocks of cell-cell adhesion
Pakorn Tony Kanchanawong
Nanoscale Structure-Function Relationship, Super-resolution Microscopy, Cell-Matrix Interactions, Cell-Cell Interactions, Cellular Biophysics, Mechanobiology of Cell Adhesions and Cell Cortex, Computer Vision, Machine Learning, Bioimaging Technology, Bioimage Informatics.
Cells built complex nanoscale ‘machines’ from basic biomolecular building blocks to perform vital biological functions. These cellular ‘machines’ are at the heart of key processes in mechanobiology, such as cell migration, cell adhesion, and mechanotransduction. Our overarching research goal is to gain a comprehensive insight into the nanoscale structure-function relationship that governs the assembly, organization, dynamics, and functions of these cellular machines. Our approach is highly interdisciplinary, combining advanced imaging technologies with rigorous molecular and cell biology methods.
We have pioneered the use of superresolution microscopy to elucidate nanoscale architecture of cellular structures (Kanchanawong et al., Nature, 2010), and have long-standing involvement in the development of ultra-high resolution 3D imaging techniques, iPALM (Shtengel et al., PNAS 2009). Our focus is in advancing the capability of super-resolution microscopy, using several platforms including our own iPALM system and surface-generated structured illumination techniques.
Super-resolution and advanced microscopy techniques generate beautiful, complex, and exquisitely detailed images of cells in large quantity. These images contain vast amount of information but it is still very challenging to quantitatively, rigorously, and comprehensively analyse such datasets. To fully tap the potential of these 21st century imaging techniques, this analysis bottleneck must be tackled. We have several ongoing projects where we seek to leverage computer vision and machine learning approaches to unlock information contained in super-resolution microscopy images (for example: Zhang et al., MBoC 2017).
Focal adhesions are major cell adhesion structures that mediate cell-extracellular matrix (ECM) adhesions. Focal adhesions play essential roles in mechanotransduction, rigidity sensing, and cell migration. Our focus is in understanding the molecular architecture of focal adhesions (Kanchanawong et al., Nature, 2010) and how they are animated during cellular functions. Our recent work established the roles of the protein Talin as the determinant of focal adhesions architecture (Liu et al., PNAS 2015). Ongoing projects seek to combine nanoscale imaging with molecular engineering approaches to understand the operational principles that control focal adhesions structure and functions.
In tissues, coherent organization of cells depends on cadherin-mediated cell-cell junctions. We have recently elucidated for the nanoscale architecture of cadherin-based cell adhesions, using superresolution microscopy (Bertocchi et al., Nature Cell Biology, 2017; Wu et al., Developmental Cell, 2015). In our ongoing projects we seek to understand comprehensively the transformation and linkages between nanoscale structures and functions during the formation and maturation of epithelial tissues
PhD (Biophysics) Stanford University
Tony Kanchanawong received his Bachelor’s degree (A.B. summa cum laude, 2001) from Cornell University where he double-majored in Chemistry and Biological Sciences. At Cornell, he also studied the Molecular Dynamics of cellulase enzymes in the laboratory of Prof. John W. Brady Jr. Going west to Stanford University, he worked on Non-Classical Stark spectroscopy of photosynthetic reaction centers and GFPs with Prof. Steven G. Boxer, supported by the HHMI Predoctoral Fellowship. In 2007, he received his doctorate in Biophysics and became a postdoctoral fellow in the laboratory of Dr. Clare Waterman at NIH, where he closely collaborated with Dr. Harald Hess at HHMI Janelia Research Campus in the development and application of iPALM 3-D superresolution microscopy. In 2011, Tony started his research group at MBI and NUS Department of Biomedical Engineering, as one of the NRF fellowship recipients.
- Thumkeo D, Katsura Y, Nishimura Y, Kanchanawong P, Tohyama K, Ishizaki T, Kitajima S, Takahashi C, Hirata T, Watanabe N, Krummel MF, and Narumiya S. mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse. Sci Adv 2020; 6(1):eaay2432. [PMID: 31911947]
- Wang Y, Barnett SFH, Le S, Guo Z, Zhong X, Kanchanawong P, and Yan J. Label-free Single-Molecule Quantification of Rapamycin-induced FKBP-FRB Dimerization for Direct Control of Cellular Mechanotransduction. Nano Lett. 2019; 19(10):7514-7525. [PMID: 31466449]
- Xia S, Lim YB, Zhang Z, Wang Y, Zhang S, Lim CT, Yim EKF, and Kanchanawong P. Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells. Cell Rep 2019; 28(5):1251-1267.e7. [PMID: 31365868]
- . https://www.ncbi.nlm.nih.gov/pubmed/31147615
- Rafiq NBM, Nishimura Y, Plotnikov SV, Thiagarajan V, Zhang Z, Shi S, Natarajan M, Viasnoff V, Kanchanawong P, Jones GE, and Bershadsky AD. A mechano-signalling network linking microtubules, myosin IIA filaments and integrin-based adhesions. Nat Mater 2019; 18(6):638-649. [PMID: 31114072]
- Tsai Y, Tang W, Low CSL, Liu Y, Wu J, Lee P, Chen LQ, Lin Y, Kanchanawong P, Gao L, and Chen B. Rapid High Resolution 3D Imaging of Expanded Biological Specimens with Lattice Light Sheet Microscopy. Methods 2019;. [PMID: 30978505]
- Sakamoto S, Thumkeo D, Ohta H, Zhang Z, Huang S, Kanchanawong P, Fuu T, Watanabe S, Shimada K, Fujihara Y, Yoshida S, Ikawa M, Watanabe N, Saitou M, and Narumiya S. mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. PLoS Biol. 2018; 16(9):e2004874. [PMID: 30256801]
- Ramanujam R, Han Z, Zhang Z, Kanchanawong P, and Motegi F. Establishment of the PAR-1 cortical gradient by the aPKC-PRBH circuit. Nat. Chem. Biol. 2018;. [PMID: 30177850]
- Koe CT, Tan YS, Lönnfors M, Hur SK, Low CSL, Zhang Y, Kanchanawong P, Bankaitis VA, and Wang H. Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II. Elife 2018; 7. [PMID: 29482721]
- Zhang Z, Lim YW, Zhao P, Kanchanawong P, and Motegi F. ImaEdge: a platform for the quantitative analysis of cortical proteins spatiotemporal dynamics during cell polarization. J. Cell. Sci. 2017;. [PMID: 29113997]