LOW Boon Chuan
Associate Professor, Mechanobiology Institute, National University of Singapore
+65 6516 7834 ext 67834
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
The Cell-Matrix and Cell-Cell Mechanotransduction Group
Yan WU PhD Student, Mechanobiology Institute, National University of Singapore firstname.lastname@example.org Level 10 T-Lab National University of Singapore 5A Engineering Drive 1 Singapore 117411 Yan Wu PhD Student Principal Investigator Low [...]
How cancer cells spread in response to physical cues from their surroundings
To grow or not to grow?
Molecular response to depth sensing differs in cancer cells
Low Boon Chuan
To identify novel signaling proteins and protein domains that control cell morphogenesis, motility, differentiation and cell growth and tissue/organ development during normal and disease states. Areas of interest include cell signaling, domain-discovery, protein-protein interaction, structural biology, developmental biology, computational biology and mechanobiology.
Molecular recognition forms the basis for all cellular events- from a simple bimolecular enzymatic reaction to the cascades of multimeric protein complex in cell signaling. Fundamental to the structure and function of a protein is its ‘domain’- a discrete, minimal modular entity that constitutes one of the basic physical and functional unit of the polypeptide. This protein domain can either serve as a protein docking/interaction site or an active enzymatic unit. With the emphasis on functional genomics, it is important to address what role does each of these domains play and how their potential functions can be regulated across molecular, cellular and tissue levels.
One of the several protein domains that our group first identified and characterised is a novel protein domain termed BCH domain which play important roles in regulating cell growth/death, differentiation, migration, and tissue/organ development. Based on the prototypical BNIP-2 and BPGAP1 proteins, we show that distinct BCH domains could act as key modulators for Rho and Ras small GTPases as well as their immediate regulators such as guanine nucleotide exchange factors and GTPase-activating proteins. Current effort is geared towards understanding how cells and tissues respond to the dynamic forces and geometry in the environment both under the influence of the BCH domain. This will be addressed under the newly established MBI.
Assoc Prof Low left Kuala Lumpur, Malaysia for Dunedin, New Zealand in early 90’s to pursue his dream as a scientist. Having spent wonderful undergraduate and postgraduate years at the Department of Biochemistry, University of Otago, he joined IMCB, Singapore and then NUS, trying to figure out what exactly make cells work. Amazing as it is, we are still far from understanding the intricacy underlying these processes. Trained as a biochemist, practising mainly as a cell biologist now, and with exciting arrays of multi-disciplinary tools, his team and collaborators aim to systematically unravel some of the uncharted paths, and are ready to expect the unexpected.
- Chaudhuri PK, Low BC*, Lim CT* (2018) Mechanobiology of tumor growth. Chemical Reviews (in press). *co-correspondence authors.
- He MY, Xu SB, Qu ZH, Guo YM, Liu XC, Cong XX, Wang JF, Low BC, Li L, Wu Q, Lin P, Yan SG, Bao Z, Zhou YT, and Zheng LL. Hsp90β interacts with MDM2 to suppress p53-dependent senescence during skeletal muscle regeneration. Aging Cell 2019;:e13003. [PMID: 31313490]
- Chen T, Callan-Jones A, Fedorov E, Ravasio A, Brugués A, Ong HT, Toyama Y, Low BC, Trepat X, Shemesh T, Voituriez R, and Ladoux B. Large-scale curvature sensing by directional actin flow drives cellular migration mode switching. Nat Phys 2019; 15:393-402. [PMID: 30984281]
- Fan W, Gao XK, Rao XS, Shi YP, Liu XC, Wang FY, Liu YF, Cong XX, He MY, Xu SB, Shen WL, Shen Y, Yan SG, Luo Y, Low BC, Ouyang H, Bao Z, Zheng LL, and Zhou YT. Hsp70 interacts with MAPK-activated protein kinase 2 to regulate p38MAPK stability and myoblast differentiation during skeletal muscle regeneration. Mol. Cell. Biol. 2018;. [PMID: 30275345]
- Chaudhuri PK, Low BC, and Lim CT. Mechanobiology of Tumor Growth. Chem. Rev. 2018;. [PMID: 29927236]
- Ashraf S, Kudo H, Rao J, Kikuchi A, Widmeier E, Lawson JA, Tan W, Hermle T, Warejko JK, Shril S, Airik M, Jobst-Schwan T, Lovric S, Braun DA, Gee HY, Schapiro D, Majmundar AJ, Sadowski CE, Pabst WL, Daga A, van der Ven AT, Schmidt JM, Low BC, Gupta AB, Tripathi BK, Wong J, Campbell K, Metcalfe K, Schanze D, Niihori T, Kaito H, Nozu K, Tsukaguchi H, Tanaka R, Hamahira K, Kobayashi Y, Takizawa T, Funayama R, Nakayama K, Aoki Y, Kumagai N, Iijima K, Fehrenbach H, Kari JA, El Desoky S, Jalalah S, Bogdanovic R, Stajić N, Zappel H, Rakhmetova A, Wassmer S, Jungraithmayr T, Strehlau J, Kumar AS, Bagga A, Soliman NA, Mane SM, Kaufman L, Lowy DR, Jairajpuri MA, Lifton RP, Pei Y, Zenker M, Kure S, and Hildebrandt F. Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment. Nat Commun 2018; 9(1):1960. [PMID: 29773874]
- Cong XX, Rao XS, Lin JX, Liu XC, Zhang GA, Gao XK, He MY, Shen WL, Fan W, Pioletti D, Zheng LL, Liu HH, Yin Z, Low BC, Schweitzer R, Ouyang H, Chen X, and Zhou YT. Activation of AKT-mTOR Signaling Directs Tenogenesis of Mesenchymal Stem Cells. Stem Cells 2018;. [PMID: 29315990]
- Chaudhuri PK, Pan CQ, Low BC, and Lim CT. Differential Depth Sensing Reduces Cancer Cell Proliferation via Rho-Rac-Regulated Invadopodia. ACS Nano 2017;. [PMID: 28654281]
- Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, Jiang T, Low BC, Singh H, Lim CT, and Sudol M. YAP Regulates Actin Dynamics through ARHGAP29 and Promotes Metastasis. Cell Rep 2017; 19(8):1495-1502. [PMID: 28538170]
- Gupta K, Li Q, Fan JJ, Fong ELS, Song Z, Mo S, Tang H, Ng IC, Ng CW, Pawijit P, Zhuo S, Dong C, Low BC, Wee A, Dan YY, Kanchanawong P, So P, Viasnoff V, and Yu H. Actomyosin Contractility Drives Bile Regurgitation as an Early Response During Obstructive Cholestasis. J. Hepatol. 2017;. [PMID: 28189756]
- Jiang T, Pan CQ, and Low BC. BPGAP1 spatially integrates JNK/ERK signaling crosstalk in oncogenesis. Oncogene 2017;. [PMID: 28092672]