Linda J KENNEY
Professor, Mechanobiology Institute, National University of Singapore
+65 6601 1285 ext 11285
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Bacterial Pathogenesis and Signal Transduction Lab (BPAST)
Molecular Mechanics of Mechanotransduction
A bacterial molecular switch between virulence or dormancy
A gene-silencing nucleoprotein filament formed by H-NS family proteins
How does Salmonella thrive in the acidic macrophage?
Linda J Kenney
Signal transduction in Bacteria; Bacterial Pathogenesis; Mechanotransduction and osmotic signaling in E. coli; Mechanisms of anti-silencing of virulence genes in Salmonella
Our laboratory is interested in signal transduction and the regulation of gene expression in prokaryotes. In particular, we are studying the two-component regulatory system EnvZ/OmpR that regulates the expression of outer membrane proteins as well as many other genes. Our present work focuses on how OmpR activates genes required for systemic infection (located on Salmonella pathogenicity island 2) in Salmonella enterica.
The Kenney Lab’s research was recently featured in an article exploring the bacterial molecular switch between virulence or dormancy, Salmonella Lifestyle Choices. Members of her lab have discovered that the bacterial protein SsrB is the molecular switch for determining whether Salmonella infections become acute and virulent, or remain in a dormant carrier state.
The study is published in eLife (Desai et al., The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing, February 2, 2016, eLife 2016; 5: e10747, doi: 10.7554/eLife.10747). Read full article.
Dr Kenney is a Professor of Microbiology at the University of Illinois-Chicago. Her laboratory studies two-component systems in bacteria that control gene expression at a single cell and nanometer level.
PhD University of Pennsylvania
- Lin M, Zhang G, Fahie M, Morgan LK, Chen M, Keiderling TA, Kenney LJ, and Liang J. Engineering a Novel Porin OmpGF via Strand Replacement From Computational Analysis of Sequence Motif. Biochim. Biophys. Acta 2017;. [PMID: 28341438]
- Ghosh M, Wang LC, Ramesh R, Morgan LK, Kenney LJ, and Anand GS. Lipid-Mediated Regulation of Embedded Receptor Kinases via Parallel Allosteric Relays. Biophys. J. 2017; 112(4):643-654. [PMID: 28256224]
- Desai SK, and Kenney LJ. To ∼P or Not to ∼P? Non-canonical Activation by Two-Component Response Regulators. Mol. Microbiol. 2016;. [PMID: 27656860]
- Desai SK, Winardhi RS, Periasamy S, Dykas MM, Jie Y, and Kenney LJ. The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. Elife 2016; 5. [PMID: 26880544]
- Winardhi RS, Yan J, and Kenney LJ. H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments. Biophys. J. 2015; 109(7):1321-9. [PMID: 26445432]
- Foo YH, Spahn C, Zhang H, Heilemann M, and Kenney LJ. Single cell super-resolution imaging of E. coli OmpR during environmental stress. Integr Biol (Camb) 2015; 7(10):1297-308. [PMID: 26156621]
- Foo YH, Gao Y, Zhang H, and Kenney LJ. Cytoplasmic sensing by the inner membrane histidine kinase EnvZ. Prog. Biophys. Mol. Biol. 2015; 118(3):119-29. [PMID: 25937465]
- Chakraborty S, Mizusaki H, and Kenney LJ. A FRET-based DNA biosensor tracks OmpR-dependent acidification of Salmonella during macrophage infection. PLoS Biol. 2015; 13(4):e1002116. [PMID: 25875623]
- Cheong FC, Wong CC, Gao Y, Nai MH, Cui Y, Park S, Kenney LJ, and Lim CT. Rapid, high-throughput tracking of bacterial motility in 3D via phase-contrast holographic video microscopy. Biophys. J. 2015; 108(5):1248-56. [PMID: 25762336]
- Lim CJ, Kenney LJ, and Yan J. Single-molecule studies on the mechanical interplay between DNA supercoiling and H-NS DNA architectural properties. Nucleic Acids Res. 2014; 42(13):8369-78. [PMID: 24990375]