About the National University of Singapore

About NUSA leading global university centred in Asia, NUS is Singapore's flagship university, offering a global approach to education and research with a focus on Asian perspectives and expertise.

About the Mechanobiology Institute, National University of Singapore

About MBIOne of four Research Centres of Excellence at NUS, MBI is working to identify, measure and describe how the forces for motility and morphogenesis are expressed at the molecular, cellular and tissue level.
Microscopy Core 2017-05-03T10:10:56+00:00

MBI’s Microscopy Facility

The Microscopy Core is the largest of the MBI core facilities, serving as the backbone for most of the experimental work at the Institute. The Core supports image related research, offering a wide range of microscopes and image analysis resources.

Visualization of the dynamic biological processes rely on advanced imagining technologies. The Microscopy Core is the largest of the MBI core facilities, serving as the backbone for most of the experimental work at the Institute. The Core supports image related research, offering a wide range of microscopes and image analysis resources.

L to R: Peng Qiwen, Bi Feng, Felix Margadant, Bai Funing, Mak Kah Jun, Ong Hui Ting

Instrumentation

As of 2010, MBI’s microscopy facility has the following instruments:

Three confocals specified for different tasks: a PerkinElmer Spinning disk for very high speed confocal imaging at lowest light levels, a NikonA1Rsi equipped with a 32 channel spectral imaging head, the Zeiss Conforcor III for fluorescent correlation spectroscopy with ultra-low chromatic aberration lenses and the newest avalanche diode sensors offering triple the sensitivity of high end FCS system.

MBI provides equipment, staff and services to support and overcome the experimental challenges that our PIs, researchers  and students face.

Three wide-field fluorescence multi-position live cell imaging systems for long term imaging: the DeltaVision Core which offers true 3D widefield imaging and automated time series of volume datasets on several specimen all in a full sized environmental chamber, the Olympus Live Cell imaging system with a cooled HQ+ CCD camera and a full size incubation chamber, HBO lines, and a motorized stage, and the Nikon BiostationIMQ which offers automated single specimen operation.

Three TIRF based microscopes optimized for different purposes: the Olympus TIRF for multiwavelength TIRF, hosting two high end EMCCD camera systems and a motorized stabilized stage in a full size clear chamber for single photon counting and high resolution imaging at lowest light conditions useful for long time single molecule observation. The Zeiss Elyra Beta with high power solid state laser modules and an EMCCD camera system for super resolution imaging (PALM and STORM) in a full size enclosed chamber, and the Nikon TIRF with a compact EMCCD camera for magnet tweezer operation.

One motorized stereomicroscope for the microfabrication Core, one AFM, and one magnetic piezo tweezer system.

For image processing, we have Huygens for deconvolution, Imaris and Volocity for 3D visualization and data analysis, MetaMorph and ImageJ for data analysis, and three high end workstations sporting each 8 cores, 48GB, 4TB RAID0 disk systems, and fast graphics on 30” screens.

Learn More

The Microscopy Core currently has three full-time staff providing training and consultation to MBI and NUS researchers. Internal staff may learn more about the Core,  including calendaring and booking, a complete workshop schedule, user policy and safety, and equipment overview at the MBI Microscopy Core site.

Location

mbi@nus.edu.sg
+65 6872 6123
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

MARGADANT, Felix

Facility Manager
mbifmm@nus.edu.sg

LIU Jun

LIU Jun

Superresolution, FCS
mbilj@nus.edu.sg

CHIN Fei Li, Jasmine

CHIN Fei Li, Jasmine

TIRF, Spinning Disk
mbicflj@nus.edu.sg

MAK Kah Jun

Superresolution, TIRF, STORM chemistry
mbimkj@nus.edu.sg

BAI Funing

Image Processing
mbibf@nus.edu.sg

ONG Hui Ting

Image Processing
mbioht@nus.edu.sg

Peng Qiwen

Image Processing
mbipeng@nus.edu.sg

BI Feng

Image Processing
@nus.edu.sg