MBI’s Protein Cloning Core

The purification and expression of proteins enable cutting-edge molecular and cellular research here at the Mechanobiology Institute. The core provides fast, efficient cloning and expression services to researchers at MBI and NUS.

The purification and expression of proteins enable cutting-edge molecular and cellular research here at the Mechanobiology Institute. The Protein Cloning and Expression Core provides fast, efficient cloning and expression services to researchers at MBI and NUS.

Managed by four full-time research staff, the Core provides high-quality, purified recombinant proteins from different host systems (E.coli, yeast, insect and mammalian) and custom-made expression vectors with selective markers. The final products are characterized by their purity, promoting and assuring accuracy for quality research. The Core also provides consultation services and training for new staff inexperienced in molecular biology and protein purification protocols.

A Collection of Fluorescent Proteins with Different Colors

The MBI Protein Cloning and Expression Core has collected and/or constructed a series of fusion vectors, which provide the opportunities to label and trace the target genes in vivo under microscope.

The core has developed high-throughput “in-fusion” approaches to label any target genes with different colors, ranging from green, yellow, red to cherry. In addition, the core facility is developing the new approaches to make novel fluorescent proteins with “tailor-made” colors based on structures.

Services

Protein Expression Facility [NUS and MBI researchers only]

Researchers at NUS and MBI may access the Protein Expression Facility for training and scheduling information and to submit project requests (login required).

MBI’s Internal Component Repository (OpenFreezer)

MBI’s install of OpenFreezer is an online repository collecting and presenting information about the plasmids/clones/drugs/antibodies/chemicals and other resources internally available at MBI. Administered by MBI’s Wet Lab Core, the goal of the resource is to facilitate better communication and more efficient sharing of components among the different groups within MBI. Users may submit cloning and protein expression requests via OpenFreezer to MBI’s Protein Cloning Core. Learn more

Location

mbi.nus.edu.sg
mbi@nus.edu.sg
+65 6872 6123

Mechanobiology Institute, National University of Singapore
Level 5 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Campus map

CHEN Hongying

mbichy@nus.edu.sg

Paramasivam KATHIRVEL

mbipk@nus.edu.sg