Assistant Professor, Department of Biomedical Engineering and Department of Surgery, National University of Singapore
Interaction of adult and embryonic stem cells with nanotopography; Nanotopography induced differentiation of adult and embryonic stem cells for neural, cornea and vascular tissue engineering; Regulation of stem cells lineage commitment and differentiation; Biomaterial approach to study ex-vivo stem cell expansion
Stem cells hold great potential in tissue engineering whereby diseased or damaged tissue can be regenerated through a selective yet intricate combination of scaffold, growth factors and cells. The success of tissue engineering approaches however, relies upon a systematic regulation of stem cell growth and differentiation in vitro for a viable and ready cell source. In contrast to classical regulation of stem cell fate using chemical modulators, physical attributes of the extracellular matrix has been shown to be crucial mediators of stem cell fate. Using cellular differentiation as a model, we seek to understand the molecular basis of stem cells’ ability to sense the extracellular architecture and the transmission of these mechanical signals into a lineage regulation mechanism. These findings will be instrumental in efficient stem cell fate regulation and also understanding diseases involving mechanical dysfunction, some of which includes cancer and genetic malformations.
Prof Yim received her Ph.D. in the Biomedical Engineering at the Johns Hopkins University before undergoing her Post-doctoral training at the Johns Hopkins School of Medicine and in the Department of Biomedical Engineering at Duke University before she joined the National University of Singapore as a faculty in the Division of Bioengineering and Department of Surgery in 2007. Her research interests in understanding how the chemical and biomechanical cues influence stem cells behaviour spurred her to join MBI in 2009, together with a group of akin researchers focused on cell and tissue mechanics. Experienced with nanofabrication technologies and stem cell culture techniques, Evelyn will lead her group in MBI in studying the interaction between cell and the biomechanical cues in the microenvironment, and to apply this knowledge to direct stem cell differentiation and tissue regeneration.
Cutiongco MFA, Peneyra JL, Kukumberg M, Yeo MS, Yao JY, Rufaihah AJ, Le Visage C, Ho JP, Yim EKF. “Study of small diameter poly(vinyl alcohol) vascular graft in multi-level peripheral arterial occlusive disease”. Tissue Engineering Part A. Under review
Muhammad R*, Lim SH*, Goh SH, Law JBK, Saifullah MS, Ho GW, Yim EKF. “Sub-100 nm patterning of TiO2 film for the regulation of endothelial and smooth muscle cell functions”. Biomaterials Science. 2: 1740-1749. 2014.
Li K*, Yamamoto M*, Chan SJ, Chiam MY, Qin W, Wong PTH^, Yim EKF^, Tang BZ, Liu B^. “Organic nanoparticles with aggregation-induced emission for tracking bone marror stromal cells in rat ischemic stroke model.” Chemical Communications. 50: 15136-15139. 2014.
Wong ST, Teo SK, Park S, Chiam KH and Yim EKF. “Anisotropic rigidity sensing on grating topography directs human mesenchymal stem cell elongation”. Biomechanics and Modeling in Mechanobiology. 13 (1): 27-39. 2014
Cutiongco MFA, Tan MH, Ng MYK, Le Visage C, Yim EKF. “Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: platform with enhanced cell interaction and spatial distribution”. Acta Biomateriala. 2014 Jun 28. pii: S1742-7061(14)00285-2
Chua JS, Chng C-P, Moe AAK, Tann JY, Goh EL, Chiam K-H, Yim EKF. “Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance”. Biomaterials. 2014 Sep;35(27):7750-61
Koo S, Muhammad R, Peh GS, Mehta JS, Yim EKF. “Micro- and nanotopography with extracellular matrix coating modulate human corneal endothelial cell behavior.” Acta Biomaterialia. 2014 May;10(5):1975-84
Muhammad R*, Lim SH*, Goh SH, Law JBK, Saifullah MS, Ho GW, Yim EKF. “Sub-100 nm patterning of TiO2 film for the regulation of endothelial and smooth muscle cell functions”. Biomaterials Science, 2014, Advance Article
Teo BKK, Tan GD, Yim EKF. “The synergistic effect of nanotopography and sustained dual release of hydrophobic and hydrophilic neurotrophic factors on human mesenchymal stem cell neuronal lineage commitment.” Tissue Eng Part A. 2014 Aug;20(15-16):2151-61
Chua JS, Liew LX, Yim EKF. “Cultivation of Human Microvascular Endothelial Cells on Topographical Substrates to Mimic the Human Corneal Endothelium.” Journal of Functional Biomaterials. 4(1), 38-58. 2013.
Ino J, Sju E, Yim EKF, Letourneur D and Le Visage C, “Evaluation of hemocompatibility and endothelialization of hybrid polyvinyl alcohol (PVA)/gelatin polymer films.” Journal of Biomedical Materials Research: Part B – Applied Biomaterials. 101(8): 1549-1559. 2013.
Ankam S, Teo BKK, Kukumberg M, Yim EKF. “High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment.” Organogenesis. 9 (3): 128-142. 2013.
Teo BKK, Wong ST, Lim CK, Kung TYS, Yap CH, Ramagopal Y, Romer LH, Yim EKF. “Nanotopography Modulates Mechanotransduction of Stem Cells and Induces Differentiation through Focal Adhesion Kinase” ACS Nano. 7(8): 4785-4798. 2013.
Chan LY, Yim EKF, Choo ABH. “Normalized Median Fluorescence: An Alternative Flow Cytometry Analysis Method for Tracking Human Embryonic Stem Cell States During Differentiation“. Tissue Engineer Part C Methods. 19 (2), 156-165. 2013.
Ankam S, Suryana M, Chan LY, Moe, AAK, Teo BKK, Law JBK, Sheetz MP, Low HY, Yim EKF. “Substrate topography and size determines the fate of human embryonic stem cells to neuronal or glial lineage”. Acta Biomaterialia. 9 (1), 4535–4545. 2013.
Chan LY, Birch WR, Yim EKF*, Choo ABH*. “Temporal Application of Topography to Increase the Rate of Neural Differentiation from Human Pluripotent Stem Cells”. Biomaterials. 34: 382-392. 2013 *co-corresponding authors
Yim EKF and Sheetz MP. “Force dependent cell signaling in stem cell differentiation”. Stem Cell Research and Therapy. 3 (5): 38-40. 2012.
Teo BKK, Goh KJY, Ng ZJ, Koo S and Yim EKF. “Functional reconstruction of corneal endothelium using nanotopography for tissue-engineering applications.” Acta Biomaterialia. 8 (8):2941-2952. 2012.
Moe AAK, Suryana M, Marcy GT, Lim SK, Ankam S, Goh JZW, Jin J, Teo BKK, Law JBK, Low HY, Goh ELK, Sheetz MP and Yim EKF. “Microarray with micro- and nano-topographies enables identification of optimal topography to direct differentiation of primary murine neural progenitor cells”. Small. 8(19):3050-61. 2012
Yow SZ, Lim TH, Lim CT, Yim EKF, Leong KW. “A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering.” Polymers. 3: 527-544. 2011.
Koo S*, Ahn SJ*, Zhang H, Wang JC and Yim EKF. “Human Corneal Keratocyte Response to Micro- and Nano-gratings on Chitosan and Polydimethylsiloxane.” Cellular and Molecular Bioengineering. 4 (3): 399-410. 2011. *co-first authors
Teo BKK*, Goh SH*, Kustandi TS, Loh WW, Low HY, Yim EKF. “The effect of micro and nanotopography on endocytosis in drug and gene delivery systems.” Biomaterials. 32 (36): 9866-9875. 2011. *co-first authors
Teo BKK, Ankam S, Chan LY and Yim EKF. “Nanotopography/mechanical induction of stem-cell differentiation.” Methods Cell Biology. 98: 241-94. 2010.
Yim EKF, Darling EM, Kulangara K, Guilak F and Leong KW. “Nanotopography-induced changes in focal adhesions, cytoskeletal organization, and mechanical properties of human mesenchymal stem cells.” Biomaterials. 31 (6): 1299-1306, 2010.