About the National University of Singapore

About NUSA leading global university centred in Asia, NUS is Singapore's flagship university, offering a global approach to education and research with a focus on Asian perspectives and expertise.

About the Mechanobiology Institute, National University of Singapore

About MBIOne of four Research Centres of Excellence at NUS, MBI is working to identify, measure and describe how the forces for motility and morphogenesis are expressed at the molecular, cellular and tissue level.
Yan Jie 2017-05-03T10:39:30+00:00
YAN Jie

YAN Jie

Professor, Mechanobiology Institute, National University of Singapore

phyyj@nus.edu.sg
+65 6516 2620 ext 62620
Level 10 T-Lab
National University of Singapore
5A Engineering Drive 1
Singapore 117411

Laboratory website
Yan Jie Group Single Molecule Biophysics

Research Program
Molecular Mechanisms of Mechanobiology Group

Affiliations
Professor, Department of Physics, National University of Singapore

Professor, Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore

Recent Research

Yan Jie

Principal Investigator

Research Areas

Stability and Interaction of Biomolecules under Mechanical Constraints

Research Interests

  • Force-dependent conformations and interactions of nucleic acids and proteins
  • Mechanosensing of cells at cell-ECM adhesion and cell-cell junction
  • Mechanisms of chaperone-mediated protein folding
  • Chromosome packaging, gene regulations, and DNA damage repair

Biography

While being trained in theoretical physics, Dr Jie Yan built a strong interest in studying molecular cell biology using a combination of novel single-molecule biophysical methods and theoretical modeling. Since he joined the Department of Physics of NUS in 2005, he has been leading a group to study DNA-protein interactions and micromechanics of biopolymers. He became a Principal Investigator in MBI in 2009, since then he has been focusing on studies of the effects of mechanical constraints on the stability and interactions of biomolecules.

Recent Publications

  1. Yuan G, Le S, Yao M, Qian H, Zhou X, Yan J, and Chen H. Elasticity of the Transition State Leading to an Unexpected Mechanical Stabilization of Titin Immunoglobulin Domains. Angew. Chem. Int. Ed. Engl. 2017;. [PMID: 28394039]
  2. You H, Lattmann S, Rhodes D, and Yan J. RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis. Nucleic Acids Res. 2016; 45(1):206-214. [PMID: 28069994]
  3. Winardhi RS, Tang Q, Chen J, Yao M, and Yan J. Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding. Biophys. J. 2016; 111(11):2349-2357. [PMID: 27926836]
  4. Efremov AK, Winardhi RS, and Yan J. Transfer-matrix calculations of DNA polymer micromechanics under tension and torque constraints. Phys Rev E 2016; 94(3-1):032404. [PMID: 27739846]
  5. Yan J. Mechanisms of DNA disentangling by type II topoisomerases: Comment on "Disentangling DNA molecules" by Alexander Vologodskii. Phys Life Rev 2016;. [PMID: 27666769]
  6. Li W, Chen P, Yu J, Dong L, Liang D, Feng J, Yan J, Wang P, Li Q, Zhang Z, Li M, and Li G. FACT Remodels the Tetranucleosomal Unit of Chromatin Fibers for Gene Transcription. Mol. Cell 2016;. [PMID: 27666592]
  7. Kulemzina I, Ang K, Zhao X, Teh J, Verma V, Suranthran S, Chavda AP, Huber RG, Eisenhaber B, Eisenhaber F, Yan J, and Ivanov D. A Reversible Association between Smc Coiled Coils Is Regulated by Lysine Acetylation and Is Required for Cohesin Association with the DNA. Mol. Cell 2016;. [PMID: 27618487]
  8. Yao M, Goult BT, Klapholz B, Hu X, Toseland CP, Guo Y, Cong P, Sheetz MP, and Yan J. The mechanical response of talin. Nat Commun 2016; 7:11966. [PMID: 27384267]
  9. Lee SY, Lim CJ, Dröge P, and Yan J. Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF. Sci Rep 2015; 5:18146. [PMID: 26657062]
  10. Cong P, Dai L, Chen H, van der Maarel JRC, Doyle PS, and Yan J. Revisiting the Anomalous Bending Elasticity of Sharply Bent DNA. Biophys. J. 2015; 109(11):2338-51. [PMID: 26636945]

Selected Publications

  1. Yuan G, Le S, Yao M, Qian H, Zhou X, Yan J, and Chen H. Elasticity of the Transition State Leading to an Unexpected Mechanical Stabilization of Titin Immunoglobulin Domains. Angew. Chem. Int. Ed. Engl.2017.
  2. You, H., Lattmann, S., Rhodes, D., Yan, J. (2017). “RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.” Nucleic Acids Research. 46: 206-214.
  3. Yao, M., Goult, B.T., Klapholz, B., Hu, X., Toseland, C.P., Guo, Y., Cong, P., Sheetz, M., and Yan, J. (2016). ” The mechanical response of talin.” Nature Communications, 7: 11966.
  4. Winardhi, R. S., Tang, Q., Chen, J., Yao, M., and Yan, J. (2016). ” Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.” Biophysical Journal, 111(11), 2349-2357.
  5. Cong, P., Dai, L., Chen, H., van der Maarel, J.R.C., Doyle, P.S., and Yan, J. (2015). “Revisiting the Anomalous Bending Elasticity of Sharply Bent DNA.” Biophysical Journal, 109: 2338-2351.
  6. You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
  7. Chen, H., Yuan, G., Winardhi, R.S., Yao, M., Popa, I., Fernandez, J.M., Yan, J. (2015). “Dynamics of equilibrium folding and unfolding transitions of titin immunoglobulin domain under constant forces.” Journal of the American Chemical Society, 137: 3540-3546.
  8. You, H., Wu, J., Shao, F., Yan, J. (2015). “Stability and Kinetics of c-MYC Promoter G-Quadruplexes Studied by Single-Molecule Manipulation.” Journal of the American Chemical Society (Communication), 137: 2424-2427.
  9. Zhang, X., Qu, Y., Chen, H., Rouzina, I., Zhang, S., Doyle, P., Yan, J. (2014). “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136: 16073 -16080.
  10. Yao, M., Qiu, W., Liu, R. , Efremov, A.K., Cong, P., Seddiki, R., Payre, M., Lim, C.T., Ladoux, B., Me`ge, R.M., and Yan, J. (2014). “Force-dependent conformational switch of alpha-catenin controls vinculin binding.” Nature Communications, 5:4525.

Lab Members

Artem Yefremov

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Senior Research Fellow, Yan Jie Lab

Ranjit S Gulvady

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PhD Student, Yan Jie Group

Ladislav Hovan

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Yan Jie Group, Sudol Group

Guo Shiwen

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PhD Student, Yan Jie Group

Le Shi Min

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Dept of Physics, Yan Jie Group

Yu Miao

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Research Fellow, Yan Jie Group

Zhao Xiao Dan

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Research Fellow, Yan Jie Group

Liu Meihan

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Research Assistant, Yan Jie Group

Yang Kaiyuan

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Research Assistant, Yan Jie Group

Zhou Yu

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Research Assistant, Yan Jie Group

Chen Jin

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PhD Student, Yan Jie Group

Lu Chen

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PhD Student, Yan Jie Group

Tang Qingnan

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Dept of Physics, Yan Jie Group