By Hu Chen1, Saranya Chandrasekar1, Michael P. Sheetz1, 2, Thomas P. Stossel3, Fumihiko Nakamura3* and Jie Yan1, 4, 5*
The actin crosslinking protein filamin A (FLNa) mediates mechanotransduction, a conversion of mechanical forces into cellular biochemical signals to regulate cell growth and survival. To provide more quantitative insight into this process, we report results using magnetic tweezers that relate mechanical force to conformational changes of FLNa immunoglobulin-like repeats (IgFLNa) 20–21, previously identified as a mechanosensing domain.
We determined the force magnitudes required to unfold previously identified structural organizations of the β-strands in the two domains: IgFLNa 20 unfolds at ~15 pN and IgFLNa 21 unfolding requires significantly larger forces. Unfolded domain IgFLNa 20 can exist in two different conformational states, which lead to different refolding kinetics of the IgFLNa 20 and imply a significant impact on the reformation of the domain pair at reduced force values. We discuss the relevance of the findings to force bearing and mechanosensing functions of FLNa.
Read further about the research activities of Yan Jie’s group.
1Mechanobiology Institute, National University of Singapore, Singapore 117411
2 Department of Biological Sciences, Columbia University, New York, NY 10027, USA
3Translational Medicine Division, Dept of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA
4Department of Physics, National University of Singapore, Singapore 117542
5 Centre for Bioimaging Sciences, National University of Singapore, Singapore 117546