Assistant Professor, Department of Biomedical Engineering, National University of Singapore
The scientific interest of our lab is at the interface of cell biology, biophysics, and advanced imaging technology. We are interested in developing and applying superresolution microscopy to study how proteins are assembled into nanoscale machinery within the cells, with current focus on the integrin-based adhesion complexes (focal adhesions).
The resolution of conventional fluorescence microscope is limited by diffraction to ~250 nm. The aim of super-resolution microscopy is to combine the exquisite sensitivity afforded by fluorescence with high spatial resolution in the nanometer scale, comparable to electron microscope.
We are interested in advancing methodologies and applications of superresolution microscopy through both optical instrumentation and computational approaches. We are building an iPALM instrument for 3-dimensional superresolution microscopy at the MBI and will use this platform for live cell imaging, and as an optical research platform. Some example iPALM images can be found here.
Integrin-mediated cell adhesions
Cell adhesion to the extracellular matrix (ECM) is mediated by integrin and numerous other signaling proteins. Integrin-based adhesions (commonly called Focal Adhesions) play essential roles in many cellular processes, such as migration, mechanotransduction, immune response. We have recently applied iPALM to decipher the molecular architecture of the Focal Adhesions, revealing for the first time a stratified organization that is spanned by talin. We plan to focus on the genesis of this architecture and the biophysical roles of the integrin receptors and large proteins during the assembly of the adhesions.
Nanoscale architecture of cellular structures
We have a broad interest in applying super-resolution microscopy to visualize the nanoscale architecture of subcellular structures. In addition to our ongoing interest is in the Focal Adhesions systems, we are open to collaboration to apply super-resolution techniques to other biological systems.
2007 Ph.D. (Biophysics) Stanford University
2001 A.B. Summa cum laude (Chemistry and Biological Sciences) Cornell University
Cristina Bertocchi, Yilin Wang, Andrea Ravasio, Yusuke Hara, Yao Wu, Talgat Sailov, Michelle A. Baird, Michael W. Davidson, Ronen Zaidel-Bar, Yusuke Toyama, Benoit Ladoux, Rene-Marc Mege, and Pakorn Kanchanawong. “Nanoscale architecture of cadherin-based cell adhesions” Nature Cell Biology 2016. Advance online publication
Z Zhang, Y Nishimura, P Kanchanawong “Extracting Microtubule Networks from Superresolution Single-Molecule Localization Microscopy Data” Molecular Biology of the Cell, 2016, E16-06-0421.
Wang, Y., Kanchanawong, P. “Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM).” J. Vis. Exp. (118), e54774, doi:10.3791/54774 (2016).
Wu Y, Kanchanawong P, Zaidel-Bar R: Actin-Delimited Adhesion-Independent Clustering of E-Cadherin Forms the Nanoscale Building Blocks of Adherens Junctions. Developmental Cell. 2015, in press.
Shtengel G, Wang Y, Zhang Z, Goh WI, Hess HF, Kanchanawong P: Imaging cellular ultrastructure by PALM, iPALM, and correlative iPALM-EM. Methods In Cell Biology, Elsevier (Eds. T. Wittman & J. Waters), 2014.
Bertocchi C, Goh WI, Zhang Z, Kanchanawong P. Nanoscale Imaging by Superresolution Fluorescence Microscopy and Its Emerging Applications in Biomedical Research. Critical Reviews in Biomedical Engineering, 2013, 41.4-5.
Nomachi, M. Yoshinaga, J. Liu, P. Kanchanawong, D. Thumkeo, T Watanabe, S. Narumiya, T. Hirata. Moesin Controls Clathrin-Mediated S1PR1 Internalization in T Cells. Plos ONE, 2013, 8(12): e82590.
Kanchanawong P, Waterman CM. Localization-based Super-resolution Imaging of Cellular Structures, Adhesion Protein Protocols, Methods in Molecular Biology, Humana Press, A. Coutts (ed.), 2013, 1046:59-84, .