Gianluca Grenci

portrait-gianluca-grenciSenior Research Fellow, MBI

website: Nano and Microfabrication Core

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Micro/nano engineering; Microfluidic; FTIR

Our laboratory is primarly interested in the application of micro/nano fabrication technology to biological science. We exploit standard and advanced micro-fabrication tools in order to design and produce systems and devices for cell culturing and imaging. Examples of such devices are: topographically and/or chemically micro-textured environments, microfluidic devices, micro-optical systems and more.

We are also interested in developing microfluidic devices for FTIR spectromicroscopy of living cells. FTIR is an imaging technique that is intrinsically label-free and requires minimal sample preparation; when coupled with microscopy and high brilliance IR sources it allows the acquisition of chemical maps at a resolution which is diffraction limited. Absorption of IR photons induces very low or no damage at all, therefore it is in principle possible to observe for prolonged time the behaviour of living cells. Our research activity is intended to develope microfludic platforms suitable for FTIR (key parameters are optical transparency and low IR absorption) while keeping cells alive and healthy; a beneficial feature provided by micro-fabrication approach is the possibility to control of the chemical environment at the micro-scale.

Dr. Gianluca Grenci joined MBI in 2012 as a research fellow and head of the Micro Fabrication Core facility. Previously he was employed at the LILIT micro/nano fabrication group (IOM-CNR, Trieste, IT) for a total of 6 years, during which he was mainly involved in the design and fabrication of microfluidic devices for synchrotron-light related spectroscopic techniques, such as SAXS and FTIR. He thus developed extensive practical knowledge on all the major lithographic technologies (UV and EB lithography, wet/dry etching, soft-lithography, thin films deposition), plus some less usual and/or more advanced technique, such as X-ray Lithography and LIGA.

He did his PhD in the field of applied superconductivity, in a project aimed to develop a current cryo-comparator (CCC) using high critical temperature superconductors of the cuprate family (YBCO) in the form of a thick film deposited onto a large area, complex shaped silver substrate.

[1] R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, J.B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM.,” Nat. Methods, 2015, vol. 12(7), pp. 641-4.

[2] I. Stassen, M. Styles, G. Grenci, H. Van Gorp, W. Vanderlinden, S. De Feyter, P. Falcaro, D. De Vos, P. Vereecken, and R. Ameloot, “Chemical vapour deposition of zeolitic imidazolate framework thin films,” Nat Mater, doi:10.1038/nmat4509

[3] G. Grenci, E. Sovernigo, A.Z. Khokhar, N. Gadegaard, M. Prasciolu, and M. Tormen, “Microfabrication of sharp blazed gratings by a two-step height amplification process based on soft and deep X-ray lithography”, Sensors Actuators A Phys., 2014, 205, 111-118.

[4] E. Mitri, G. Birarda, L. Vaccari, S. Kenig, M. Tormen, and G. Grenci, “SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells”, Lab Chip, 2013, 14, 210.

G. Birarda, A. Ravasio,M. Suryana,S. Maniam,H.-Y. N. Holman and G. Grenci, IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells, Lab Chip ,2016, 16,1644–1651

Stassen I, Styles M, Grenci G, Gorp HV, Vanderlinden W, Feyter SD, et al. Chemical vapour deposition of zeolitic imidazolate framework thin films. Nature Materials 15 (2016), 304–310,doi:10.1038/nmat4509

F. Cerrina and G. Grenci, X-Ray Lithography, In Reference Module in Materials Science and Materials Engineering, Elsevier, 2016, Current as of 28 October 2015, ISBN 9780128035818,

Mitri E, Kenig S, Coceano G, Bedolla DE, Tormen M, Grenci G, et al. Time-Resolved FT-IR Microspectroscopy of Protein Aggregation Induced by Heat-Shock in Live Cells. Analytical Chemistry 2015, 87(7): 3670-3677.

Grenci G, Zanchetta E, Pozzato A, Della Giustina G, Brusatin G, Tormen M. High resolution spin-on electron beam lithography resist with exceptional dry etching resistance. Applied Materials Today 2015, 1(1): 13-19.

Galland R, Grenci G, Aravind A, Viasnoff V, Studer V, Sibarita JB. 3D high-and super-resolution imaging using single-objective SPIM. Nature Methods 2015, 12(7): 641-644.

Mitri E, Birarda G, Vaccari L, Kenig S, Tormen M, Grenci G. SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells. Lab on a Chip Miniaturisation for Chemistry and Biology 2014, 14(1): 210-218.

Jark W, Grenci G. Focusing X-rays in two dimensions upon refraction in an inclined prism. Proceedings of SPIE – The International Society for Optical Engineering; 2014; 2014.
Jark W, Grenci G. Bidimensional focusing of x rays by refraction in an edge. Optics Letters 2014, 39(5): 1250-1253.

Ianeselli L, Grenci G, Callegari C, Tormen M, Casalis L. Development of stable and reproducible biosensors based on electrochemical impedance spectroscopy: Three-electrode versus two-electrode setup. Biosensors and Bioelectronics 2014, 55: 1-6.

Grenci G, Sovernigo E, Khokhar AZ, Gadegaard N, Prasciolu M, Tormen M. Microfabrication of sharp blazed gratings by a two-step height amplification process based on soft and deep X-ray lithography. Sensors and Actuators, A: Physical 2014, 205: 111-118.

Birarda G, Bedolla DE, Mitri E, Pacor S, Grenci G, Vaccari L. Apoptotic pathways of U937 leukemic monocytes investigated by infrared microspectroscopy and flow cytometry. Analyst 2014, 139(12): 3097-3106.

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