Research at the MBI

At the Mechanobiology Institute we are exploring the effects of structural micro-environments and force-induced deformations on molecules, cells and tissues in several different contexts, including the differentiation of stem cells and cancer cell pathology. The field of mechanobiology arose from the cross-roads of multiple disciplines including; cell and molecular biology, engineering, physics and computer science. New technologies such as high resolution time-lapse microscopy and micro-scale devices have vastly enhanced the study of mechanobiology, by enabling the description of complex functions at the sub-micrometer level. Learn more about research at the MBI.

Latest publications

A Microbead Supported Membrane-Based Fluorescence Imaging Assay Reveals Intermembrane Receptor-Ligand Complex Dimension with Nanometer Precision. Langmuir, 2016.

By Sruthi Jagannathan | Published June 4th, 2016

By Kabir H. Biswas1 and Jay T. Groves1,2. Langmuir. June 2016. Epub ahead of print. doi: 10.1021/acs.langmuir.6b01377. Abstract Receptor-ligand complexes spanning a cell-cell interface inevitably establish a preferred intermembrane spacing based on the molecular dimensions and orientation of the complexes. This couples molecular binding events to membrane mechanics and large-scale spatial organization of receptors on the cell…

The AP-2 Transcription Factor APTF-2 Is Required for Neuroblast and Epidermal Morphogenesis in Caenorhabditis elegans Embryogenesis. PLOS GENETICS, 2016.

By Sruthi Jagannathan | Published May 13th, 2016

By Yemima Budirahardja1, Pei Yi Tan1, Thang Doan1, Peter Weisdepp2, Ronen Zaidel-Bar1,3 PLOS GENETICS. May 2016. 12(5). e1006048. doi: 10.1371/journal.pgen.1006048. Abstract The evolutionarily conserved family of AP-2 transcription factors (TF) regulates proliferation, differentiation, and apoptosis. Mutations in human AP-2 TF have been linked with bronchio-occular-facial syndrome and Char Syndrome, congenital birth defects characterized by craniofacial deformities and…

Contribution of actin filaments and microtubules to cell elongation and alignment depends on the grating depth of microgratings. Journal of Nanobiotechnology, April 2016.

By Sruthi Jagannathan | Published April 29th, 2016

By Kyunghee Lee1, Ee Hyun Kim1,2, Naeun Oh2, Nguyen Anh Tuan1, Nam Ho Bae3, Seok Jae Lee3, Kyoung G. Lee3, Chi-Yong Eom4, Evelyn K. Yim1,5 and Sungsu Park6 Journal of Nanobiotechnology. April 2016. 14(1). 35. doi: 10.1186/s12951-016-0187-8. Abstract BACKGROUND: It has been reported that both chemical and physical surface patterns influence cellular behaviors, such as cell alignment…

Mechanics of epithelial tissues during gap closure. Current Opinion in Cell Biology, April 2016.

By Sruthi Jagannathan | Published April 28th, 2016

By Simon Begnaud1, Tianchi Chen2, Delphine Delacour1, René-Marc Mège3, Benoît Ladoux4  Current Opinion in Cell Biology. April 2016. 42. 52-62. doi: 10.1016/ Abstract The closure of gaps is crucial to maintaining epithelium integrity during developmental and repair processes such as dorsal closure and wound healing. Depending on biochemical as well as physical properties of the microenvironment, gap…

Remodeling the zonula adherens in response to tension and the role of afadin in this response. The Journal of Cell Biology, April 2016.

By Sruthi Jagannathan | Published April 25th, 2016

By Wangsun Choi1, Bipul R. Acharya2, Grégoire Peyret3, Marc-Antoine Fardin3, René-Marc Mège3, Benoit Ladoux4, Alpha S. Yap2, Alan S. Fanning5 and Mark Peifer6 The Journal of Cell Biology. April 2016. 213(2). 243-260. doi: 10.1083/jcb.201506115. Abstract Morphogenesis requires dynamic coordination between cell-cell adhesion and the cytoskeleton to allow cells to change shape and move without losing tissue integrity….

Inference of Internal Stress in a Cell Monolayer. Biophysical Journal, April 2016.

By Sruthi Jagannathan | Published April 12th, 2016

By Vincent Nier1, Shreyansh Jain2, Chwee Teck Lim3, Shuji Ishihara4, Benoit Ladoux5, Philippe Marcq6 Biophysical Journal, April 2016. 110(7). 1625-1635. doi: 10.1016/j.bpj.2016.03.002. Abstract We combine traction force data with Bayesian inversion to obtain an absolute estimate of the internal stress field of a cell monolayer. The method, Bayesian inversion stress microscopy, is validated using numerical simulations performed…

Nuclear transport of paxillin depends on focal adhesion dynamics and FAT domains. Journal of Cell Science, April 2016.

By Sruthi Jagannathan | Published April 11th, 2016

By Aneesh R. Sathe1, G.V. Shivashankar2, Michael P. Sheetz3 Journal of Cell Science. April 2016. Epub ahead of print. Abstract The nuclear transport of paxillin appears critical for paxillin function but the mechanism of transport remains unclear. We show that the nuclear transport of paxillin is tuned by focal adhesion turnover and the presence of FAT…

Two-stage sample-to-answer system based on nucleic acid amplification approach for detection of malaria parasites. Biosensors & Bioelectronics, March 2016.

By Sruthi Jagannathan | Published March 22nd, 2016

By Qing Liu1, Jeonghun Nam2, Sangho Kim3, Chwee Teck Lim4, Mi Kyoung Park1, Yong Shin5. Biosensors & Bioelectronics. March 2016. 82. 1-8. doi: 10.1016/j.bios.2016.03.050. Abstract Rapid, early, and accurate diagnosis of malaria is essential for effective disease management and surveillance, and can reduce morbidity and mortality associated with the disease. Although significant advances have been achieved for…

IR-Live: Fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells. Lab on a chip, March 2016.

By Sruthi Jagannathan | Published March 15th, 2016

By Giovanni Birarda1,2, Andrea Ravasio3, Mona Suryana3, Sivakumar Maniam3, Hoi-Ying Holman1 and Gianluca Grenci3 Lab on a chip. March 2016. doi: 10.1039/C5LC01460C. Abstract Water is a strong mid-infrared absorber, which has hindered the full exploitation of label-free and non-invasive infrared (IR) spectromicroscopy techniques for studying living biological samples. To overcome this barrier, many researchers have built sophisticated…

Chromosome intermingling-the physical basis of chromosome organization in differentiated cells. Nucleic Acids Research, March 2016.

By Sruthi Jagannathan | Published March 2nd, 2016

By Shovamayee Maharana1, K. Venkatesan Iyer1, Nikhil Jain2, Mallika Nagarajan1, Yejun Wang1 and G. V. Shivashankar3 Nucleic Acids Research. March 2016. Epub ahead of print. doi: 10.1093/nar/gkw131 Abstract Chromosome territories (CTs) in higher eukaryotes occupy tissue-specific non-random three-dimensional positions in the interphase nucleus. To understand the mechanisms underlying CT organization, we mapped CT position and transcriptional changes…