Research at the MBI

At the Mechanobiology Institute we are exploring the effects of structural micro-environments and force-induced deformations on molecules, cells and tissues in several different contexts, including the differentiation of stem cells and cancer cell pathology. The field of mechanobiology arose from the cross-roads of multiple disciplines including; cell and molecular biology, engineering, physics and computer science. New technologies such as high resolution time-lapse microscopy and micro-scale devices have vastly enhanced the study of mechanobiology, by enabling the description of complex functions at the sub-micrometer level. Learn more about research at the MBI.

Latest publications

Actomyosin contractility drives bile regurgitation as an early response during obstructive cholestasis. Journal of Hepatology, February 2017

By Sruthi Jagannathan | Published February 9th, 2017

By Kapish Gupta1, Qiushi Li2, Jun Jun Fan3, Eliza Li Shan Fong4, Ziwei Song5, Shupei Mo5, Haoyu Tang1, Inn Chuan Ng6, Chan Way Ng6, Pornteera Pawijit7, Shuangmu Zhuo8, Chen-Yuan Dong9, Boon Chuan Low10, Aileen Wee11, Yock Young Dan12, Pakorn Kanchanawong13, Peter So14, Virgile Viasnoff15, Hanry Yu16 Journal of Hepataology. February 2017. Epub ahead of print. doi: 10.1016/j.jhep.2017.01.026….

Long-range self-organization of cytoskeletal myosin II filament stacks. Nature Cell Biology, February 2017.

By Sruthi Jagannathan | Published January 23rd, 2017

By Shiqiong Hu1, Kinjal Dasbiswas2,3, Zhenhuan Guo1, Yee-Han Tee1, Visalatchi Thiagarajan1, Pascal Hersen1,4, Teng-Leong Chew5, Samuel A. Safran2, Ronen Zaidel-Bar1, Alexander D. Bershadsky1,6 Nature Cell Biology. February 2017. 19(2). 133-141. doi: 10.1038/ncb3466. Abstract Although myosin II filaments are known to exist in non-muscle cells, their dynamics and organization are incompletely understood. Here, we combined structured illumination microscopy…

BPGAP1 spatially integrates JNK/ERK signaling crosstalk in oncogenesis. Oncogene, January 2017.

By Sruthi Jagannathan | Published January 16th, 2017

By T Jiang1, C Q Pan2 and B C Low1,2,3 Oncogene. January 2017. doi: 10.1038/onc.2016.466. Epub ahead of print. Abstract Simultaneous hyperactivation of stress-activated protein kinase/c-Jun N-terminal protein kinase (SAPK/JNK) and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MEK/ERK) signaling cascades has been reported in carcinogenesis. However, how they are integrated to promote oncogenesis remains unknown. By analyzing…

Multistep nucleation of nanocrystals in aqueous solution. Nature Chemistry, January 2017.

By Sruthi Jagannathan | Published January 15th, 2017

By N.Duane Loh1,2, Soumyo Sen3, Michel Bosman4,5, Shu Fen Tan6, Jun Zhong1,2, Christian A. Nijhuis6,7, Petr Král3,8, Paul Matsudaira2,9 & Utkur Mirsaidov1,2,7,10 Nature Chemistry. January 2017. 9(1). 77-82. doi: 10.1038/nchem.2618. Abstract The nucleation and growth of solids from solutions impacts many natural processes and is fundamental to applications in materials engineering and medicine. For a crystalline solid,…

3D Protein Dynamics in the Cell Nucleus. BIOPHYSICAL JOURNAL, January 2017

By Sruthi Jagannathan | Published January 10th, 2017

By Anand P. Singh1, Remi Galland2,3, Megan L. Finch-Edmondson1,4, Gianluca Grenci1, Jean-Baptiste Sibarita2,3, Vincent Studer2,3, Virgile Viasnoff1,5,6, and Timothy E. Saunders1,5,7 Biophysical Journal. January 2017. 112(1). 133-142. doi: Abstract   The three-dimensional (3D) architecture of the cell nucleus plays an important role in protein dynamics and in regulating gene expression. However, protein dynamics within the 3D…

RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis. Nucleic Acids Research, January 2017.

By Sruthi Jagannathan | Published January 9th, 2017

By Huijuan You1, Simon Lattmann2, Daniela Rhodes3, Jie Yan4,5 Nucleic Acids Research. January 2017. 45(1). 206-214. doi: 10.1093/nar/gkw881. Abstract The DEAH-box ATP-dependent RHAU helicases specifically unfold RNA and DNA G-quadruplexes (G4s). However, it remains unclear how the RHAU’s G4 unfolding activity is coupled to different stages of the ATPase cycle. Here, using a single-molecule manipulation approach, we…

Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. The Journal of Cell Biology, January 2017

By Sruthi Jagannathan | Published January 2nd, 2017

By Nisha Bte Mohd Rafiq1,2,3, Zi Zhao Lieu1,2, Tingting Jiang1, Cheng-han Yu1, Paul Matsudaira1,2, Gareth E. Jones3, Alexander D. Bershadsky1,4 The Journal of Cell Biology. January 2017. 216(1). 181-197. doi: 10.1083/jcb.201605104 Abstract Podosomes represent a class of integrin-mediated cell-matrix adhesions formed by migrating and matrix-degrading cells. We demonstrate that in macrophage-like THP1 cells and fibroblasts stimulated to…

Nanoscale architecture of cadherin-based cell adhesions. Nature Cell Biology, December 2016

By Sruthi Jagannathan | Published December 19th, 2016

By Cristina Bertocchi1, Yilin Wang1, Andrea Ravasio1, Yusuke Hara1, Yao Wu1, Talgat Sailov1, Michelle A. Baird2, Michael W. Davidson2,3, Ronen Zaidel-Bar1,4, Yusuke Toyama1,5,6, Benoit Ladoux1,7, Rene-Marc Mege7 & Pakorn Kanchanawong1,4 Nature Cell Biology. December 2016. 19(1). 28-37. doi: 10.1038/ncb3456. Abstract Multicellularity in animals requires dynamic maintenance of cell-cell contacts. Intercellularly ligated cadherins recruit numerous proteins to form…

Coordination between intra- and extra- cellular forces regulates focal adhesion dynamics. NANO LETTERS, December 2016.

By Sruthi Jagannathan | Published December 19th, 2016

By Bibhu Ranjan Sarangi, Mukund Gupta, Bryant L Doss, Nicolas Tissot, France Lam, René Marc Mege, Nicolas Borghi, and Benoit Ladoux Nano Letters. December 2016. Epub ahead of print. doi: 10.1021/acs.nanolett.6b04364 Abstract Focal adhesions (FAs) are important mediators of cell-substrate interactions. One of their key functions is the transmission of forces between the intracellular acto-myosin network and…

Non-junctional E-Cadherin Clusters Regulate the Actomyosin Cortex in the C. elegans Zygote. Current Biology, December 2016.

By Sruthi Jagannathan | Published December 15th, 2016

By Anup Padmanabhan1, Hui Ting Ong1, Ronen Zaidel-Bar2 Current Biology. December 2016. Epub ahead of print. doi: 10.1016/j.cub.2016.10.032. Abstract Classical cadherins are well known for their essential function in mediating cell-cell adhesion via their extra-cellular cadherin domains and intra-cellular connections to the actin cytoskeleton [1-3]. There is evidence, however, of adhesion-independent cadherin clusters existing outside of cell-cell…