Benoit Ladoux

Co-Principal Investigator, MBI

email: benoit.ladoux@univ-paris-diderot.fr
lab: Cell Adhesion and Mechanics Lab

Professor, Matière et Systèmes Complexes, Université Paris Diderot & CNRS

RESEARCH AREAS
Epithelial cell migration; Microfabrication for cell mechanics; Influence of the mechanical environments on cell functions; Mechanotransduction

RESEARCH INTERESTS
Our research aims at understanding how living organisms interact with their environment. In particular, we are studying the cooperation between adhesion, biomechanical and biochemical signaling for the adaptation of living organisms to changes in their environment. To probe these questions, our laboratory has developed a repertoire of micro- and nano-fabrication tools to control and measure the chemical and mechanical environment of cells. Our research programme thus focuses on the integration of novel microfabricated devices for the quantitative imaging of living organisms.

BIOGRAPHY
With a background in Physics, Prof Ladoux started his career at Curie Institute working on single molecule biophysics. He worked on two main problems combining statistical physics, microfabrication and molecular biology: 1) chromatin condensation in real time; 2) fluctuations of a single polymer under shear flows. After a post-doc on cell mechanics, he used his knowledge in biophysics and microfabrication to start a new subject on mechanical studies of cell migration and adhesion at the University Paris Diderot. He developed original systems to characterise the mechanical interactions of cells with their migration support. He initiated a pluridisciplinary collaboration between French institutes and developed several collaborations with well-established international institutions such as IBEC (Spain), Harvard, Columbia, Carnegie Mellon (USA) and NUS. Introduced to Singapore by Prof Sheetz whom he collaborates with, he is now involved as a Principal Investigator in MBI.

SELECTED PUBLICATIONS

Strale PO, Duchesne L, Peyret G, Montel L, Nguyen T, Png E, Tampe R, Troyanovsky S, Henon S, Ladoux B, Mege RM. The formation of ordered nanoclusters controls cadherin anchoring to actin and cell–cell contact fluidity. The Journal of Cell Biology. 2015. 210(2): 333-346.

Ravasio A, Cheddadi I, Chen T, Pereira T, Ong HT, Bertocchi C, Brugues A, Jacinto A, Kabla AJ, Toyama Y, Trepat X, Gov N, Neves de Almeida L, Ladoux B. Gap geometry dictates epithelial closure efficiency. Nature Communications. 2015. 6: 7683.

Gupta M, Sarangi BR, Deschamps J, Nematbakhsh Y, Callan-Jones A, Margadant F, Mège RM, Lim CT, Voituriez R, Ladoux B. Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing. Nature Communications. 2015. 6:7525.

Vedula SR, Peyret G, Cheddadi I, Chen T, Brugués A, Hirata H, Lopez-Menendez H, Toyama Y, de Almeida LN, Trepat X, Lim CT, Ladoux B. Mechanics of epithelial closure over non-adherent environments. Nature Communications. Jan 2015. 6:6111.

Aubry D, Gupta M, Ladoux B, Allena R. Mechanical link between durotaxis, cell polarity and anisotropy during cell migration. Physical Biology. April 2015. 12(2):026008.

Hirata H, Gupta M, Vedula SR, Lim CT, Ladoux B, Sokabe M. Actomyosin bundles serve as a tension sensor and a platform for ERK activation. EMBO Reports. Frb 2015. 16(2):250-257.

Ravasio A, Vaishnavi S, Ladoux B, Viasnoff V. High-resolution imaging of cellular processes across textured surfaces using an indexed-matched elastomer. Acta Biomaterialia. March 2015. 14:53-60.

Gupta M, Kocgozlu L, Sarangi BR, Margadant F, Ashraf M, Ladoux B. Micropillar substrates: a tool for studying cell mechanobiology. Methods in Cell Biology 2015. 125:289-308.

Saw TB, Jain S, Ladoux B, Lim CT. Mechanobiology of Collective Cell Migration. Cellular and Molecular Bioengineering. March 2015. 8(1):3-13.

C. Plestant, P.-O. Strale, R. Seddiki, E. Nguyen, B. Ladoux and R.-M. Mège, N-cadherin engagement limits the recruitment of microtubules at cell-cell contacts through organization of acto-myosin, Journal of Cell Science (2014).

SRK. Vedula, H. Hirata, MH. Nai, A. Brugues, Y. Toyama, X. Trepat, C.T. Lim* and B. Ladoux*, Epithelial bridges maintain tissue integrity during collective cell migration, Nature Materials, 13, 87-96 (2014).

SRK Vedula, A. Ravasio, E. Anon, T. Chen, G. Peyret, M. Ashraf and B. Ladoux, Microfabricated environments to study collective cell behaviors, Methods in Cell Biology (Book series) 120, 235-252 (2014).

SRK. Vedula*, A. Ravasio, C.T. Lim and B. Ladoux* Collective cell migration: a mechanistic perspective, Physiology 28, 370-379 (2013).

Kevin Doxzen, SRK Vedula, Man Chun Leong, Hiroaki Hirata, Nir Gov, Alexandre J Kabla, Benoit Ladoux* and Chwee Teck Lim*, Integrative Biology 5, 2016-1035 (2013). *Corresponding authors

Y. Huang, N.-T. Nguyen, K. Seng Lok, P. Peng Foo Lee, M. Su, M. Wu, L. Kocgozlu, B. Ladoux. Multi-array cell stretching platform for high magnification real-time imaging, Nanomedecine 8, 543-53 (2013).

B. Ladoux and A. Nicolas. Physically based principles of cell adhesion mechanosensitivity in tissues. Rep. Prog. Phys. 75 (2012).

SRK. Vedula, MC. Leong, T. Lei Lai, P. Hersen, A. J. Kabla, CT. Lim* & B. Ladoux*, Emerging modes of collective cell migration induced by mechanical constraints. PNAS (2012).

E. Anon, X. Serra-Picamal, P. Hersen, N. C. Gauthier, M. P. Sheetz, X. Trepat & B. Ladoux, Cell crawling mediates collective cell migration to close undamaged epithelial gaps. Proc Natl Acad Sci U S A. 109, 10891-6 (2012).

Trichet L, Le Digabel J, Hawkins RJ, Vedula SR, Gupta M, Ribrault C, Hersen P, Voituriez R & Ladoux B., Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness, Proc Natl Acad Sci U S A. 109, 6933-6938 (2012).

M. Ghibaudo, J.-M. Di Meglio, P. Hersen & B. Ladoux. Mechanics of cell spreading within 3D-micropatterned environments. Lab on a Chip, 11, 805-812 (2011).

J. Le Digabel, N. Biais, J. Fresnais, J.-F. Berret, P. Hersen & B. Ladoux. Magnetic micropillars as a tool to govern substrate deformations. Lab on a Chip, 11, 2630-2636 (2011).

A. Saez, E. Anon, M. Ghibaudo, O. du Roure, J.-M. di Meglio, P. Hersen, A. Buguin, P. Silberzan & B. Ladoux. Traction forces exerted by epithelial cell sheets. Journal of Physics-Condensed Matter, 22, 194119 (2010).

B. Ladoux, E. Anon, M. Lambert, A. Rabodzey, P. Hersen, A. Buguin, P. Silberzan & R-M. Mège. Strength dependence of cadherins mediated adhesions. Biophysical Journal 98, 534-542 (2010).

O. du Roure, A. Saez, A. Buguin, R.H. Austin, P. Chavrier, P. Silberzan & B. Ladoux Force mapping in epithelial cell migration. Proc. Nat. Acad. Sci. USA, 102 2390-6 (2005).